Career Center
PhD Biotech Resume Example
Ting “Patrick” Chao
919-555-5555 [email protected] 123 Fake Street, Apt. 2, Durham, NC, 27706
Protein biology / Computational modeling
More than 8 years of academic lab experiences; industrial experience in Novozymes R&D and Roche R&D.
Trained and skilled in molecular biology, protein purication and spectroscopy techniques; 5 years of experience in
analyzing large data set using mathematical/computational approaches, strong problem solving and data analysis
skills; experienced in equipment operational trainings and presentations.
Skills
MOLECULAR BIOLOGY AND PROTEIN PURIFICATION
E.coli system protein expression
Molecular cloning
PCR (polymerase chain reaction)
DNA amplication and purication
Protein gel electrophoresis
Size-exclusion chromatography
Ion chromatography
HPLC(high-performance liquid chromatography)
SPECTROSCOPY AND SPECTROMETRY
UV-Vis spectroscopy
Fluorescence spectroscopy
Circular Dichroism spectroscopy
Empower software
Mass spectrometry
Amino acid analyzer
NMR (Nuclear Magnetic Resonance spectroscopy)
(protein solution 2D & high dimensional NMR)
COMPUTER SKILLS
Mathematica
R language
C++
Microsoft Ofce (Word, PowerPoint, Excel)
Research Experience
Industrial R&D Intern
ROCHE DIAGNOSTICS CORPORATION, INDIANAPOLIS, IN JUN. 2016 - AUG. 2016
Contribute analytical methods to facilitate glucose meter development
• Developed uorescence spectroscopy based methods to determine biochemistry reaction rate
• Documented potential protocol and future suggestions for rate determination
• Worked in a GXP (GLP) environment with a large team
NOVOZYMES NORTH AMERICA INC., FRANKLINTON, NC JUN. 2015 - AUG. 2015
Optimize enzyme performance for bioethanol production
• Designed a variety of experiments with members of the team
• Developed methods for protein acid hydrolysis and implementing amino acid analyzer
• Discovered the mechanisms behind current enzyme performance issues facing 2nd generation bioethanol
production
Research Assistant
DUKE UNIVERSITY, SCHOOL OF MEDICINE AUG. 2014 – DEC. 2017
Experimentally characterizing the performance of a novel drug delivery system based on ELP proteins
• Collaborated with scientists in the department of Biomedical Engineering
• Developed novel spectroscopy methods to characterize molecular structures of ELP-based drugs
• Provided further understanding of how helical peptide drugs can stay active when using ELP proteins as delivery
system
Duke Career Center • studentaffairs.duke.edu/career • 919-660-1050 •
Bay 5, Smith Warehouse, 2nd Floor • 114 S. Buchanan Blvd., Box 90950, Durham, NC 27708
Ting “Patrick” Chao
919-555-5555 [email protected] 123 Fake Street, Apt. 2, Durham, NC, 27706
DUKE UNIVERSITY, SCHOOL OF MEDICINE AUG. 2011 – DEC. 2017
Combining experimental / computational approaches to describe the mechanism of protein folding
• Routinely use high performance liquid chromatography, mass spectrometry & spectroscopy techniques
• Teamed with lab colleagues and NMR facility scientists to design and execute experiments
• Experimentally measured the exibility (protein dynamics) of a protein(λ repressor fragments) in solution
• Developed computational / biophysical models to quantitatively describe protein dynamics
• Improved the accuracy signicantly when predicting the exibility of proteins
DUKE UNIVERSITY, SCHOOL OF MEDICINE JUN. 2010 – AUG. 2011
Measuring biophysical properties of a large bacterial protein to discover its evolutional origin
• Led the design in automating repetitive uorescence spectroscopy measurements
• Implemented the automation in the collection of uorescence titration data for several projects
• Analyzed experimental data through computation modeling
• Determined the biophysical parameters (thermodynamics stability) in different parts of the protein (tandem-
repeated domains) to depict how this protein helps bacteria survive
Training and Teaching Experience
Provided equipment operational trainings on experimental instruments to students and researchers in multiple
departments
• Developed procedures for the training process on equipment operation and safety
• Designed and optimized experiments for each individual user
• Analyzed and suggested theoretical explanations for each user’s results
Led group discussions of research ethics
• Led the group members to express opinions on keeping good research ethics
• Designed materials to engage the group in active discussions
Acted as teaching assistants in 3 graduate school courses
• Assisted in Structural Methods & Structural Biochemistry I/II
• Tutored students during ofce hours to understand course materials
Education
Duke University, School of Medicine, Dept. of Biochemistry — Ph.D. Dec. 2017 (GPA 3.96)
Nankai University (China), Department of Life Science — B.S. 2010 (GPA 3.78)
Publications
• (In preparation) Hughes RG., Chao T., Oas TG., Schmidler SC. (2018). A Combined Biophysical-Statistical Model
for Interpreting Amide Hydrogen Exchange Measurements. Biophys. J.
• (Submitted) Roberts S., Schaal J., Harmon TS., Chao T., Hunt A., Miao V., Wen Y., Oas TG., Collier J., Pappu RV.,
Chilkoti A. (2017) Modulation of Order and Disorder in Recombinant Polypeptides Creates Injectable Tissue
Integrating Network. Nature
• Yang W., Chao T., Bai Y., Zhou R., Zhou W. & Bartlam M. (2010). Expression, purication, crystallization and
preliminary crystallographic analysis of PA3885 (TpbA) from Pseudomonas aeruginosa PAO1. Acta Cryst. F66,
1473-1476.
Honors and Activities
Department PhD Outstanding Poster Award (2012)
3 years of excellent scholarships of Nankai University (2006-2009)
Attended International Conference for Bio-economy, P.R.China (June 2009)
Attended 28th Annual Symposium of The Protein Society, San Diego, CA (July 2014)
Presented a poster titled “Developing NMR Methods For Predicting Residue Helicity Of MetO-λ Unfolded
State”
2 departmental seminar presentations for graduate school research
Duke Career Center • studentaffairs.duke.edu/career • 919-660-1050 • Bay 5, Smith Warehouse, 2nd Floor •
114 S. Buchanan Blvd., Box 90950, Durham, NC 27708
